Esperimental Infection of Infectious Bovine Rhinotracheitis Virus to Rabbits Immunosuppressed with Dexamethasone

Min, Won-gi; Jun, Moo-hyung; Park, Seong-kuk; An, Soo-hwan; Cha, Yeon-ho

Korean Journal of Veterinary Research 1988;28(1):105-110.

Won-gi Min¹, Moo-hyung Jun¹, Seong-kuk Park¹, Soo-hwan An², Yeon-ho Cha³

¹Department of Veterinary Medicine, College of Agriculture, Chungnam National University
²Veterinary Research Institute, Rural Development Administration
³Choongang Animal Disease Laboratory

Dexamethasone으로 면역기능(免疫機能) 저하(低下)된 토끼에 Infectious Bovine Rhinotracheitis Virus 감염시험(感染試驗)

민원기¹, 전무형¹, 박성국¹, 안수환², 차연호³

¹충남대학교 농과대학 수의학과
²농촌진흥청 가축위생연구소
³중앙가축전염병연구소

Abstract

To establish a laboratory animal model for study on development of diagnostic methods for infectious bovine rhinotracheitis virus(IBRV), experimental infection of the virus to rabbits immunosuppressed with dexamethasone(DX) were carried out. Results obtained throughout the experiments were as follows. When lymphocyte activity was measured by lymphocyte transformation to phytohaemagglutinin in parallel with total and differential leucocyte counting, both groups treated with 2.0mg DX once and 1.0mg DX daily showed marked immunosuppression between 5 to 72 hrs. after administration. The degree of suppression of lymphocyte activities was more remarkable in the latter group. IBRV PQ7 strain at $10^{7.5};TCID_{50}/0.2ml$ was inoculated into conjunctival sacs of rabbits immunosuppressed with DX and non-treated. During 3 weeks observation, the immunosuppressed groups revealed mild conjunctivitis, viremia and virus recovery by 33.3 to 100%, whereas the DX nontreated group showed viremia and virus recovery with no clinical conjunctivitis by one of three rabbits(33.3%). In conclusion, it was indicated that experimental infection of IBRV PQ7 strain to rabbit was limited in prerequisite to immunologic modification by administration of immunosuppressive drugs.