| In vitro effect of praziquantel on Heterophyopsis continua by scanning electron microscopic observation |
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Ho-choon Woo1, Myung-deuk Suh1, Sung-jong Hong2 |
1College of Veterinary Medicine, Gyeongsang National University
2College of Medicine, Gyeongsang National University |
| Heterophyopsis continua에 대한 praziquantel 시험관내 효과의 주사현미경적 관찰 |
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우호춘1, 서명득1, 홍성종2 |
1경상대학교 수의과대학
2경상대학교 의과대학 |
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| Abstract |
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This study was carried out to observe in vitro effect of praziquantel on the tegumental changes of Heterophyopsis continua with scanning electron microscope. Metacercariae were collected from the perch, Lateolabrax japonicus, by artificial digestion technique and fed to 2-week old chickens. Adult worms were recovered from small intestine of chickens 8 days after infection. For working solutions, praziquantel was diluted with TC199 medium at concentration of 0.01, 0.1. 1 and $10{mu}g/ml$. To each petri dish containing 10ml of solution, 5~10 worms were introduced and incubated at $37^{circ}C$. For the scanning electron microscopy(SEM), the worms were fixed in cold 2.5% glutaraldehyde, dehydrated in a series of graded ethanol and freeze-dried. Dried specimen was mounted on stub and coated with gold and observed in an SEM. The results were as follows: 1. Severe tegumental alterations were recognized by scanning electron microscope. Bleb formation of tegument was observed in 5 minute group and most pronounced on anterior tegument of worms. The number and size of blebs increased as incubation time prolonged. 2. The surface destruction was more pronounced at ventral margin between the oral and the ventral suckers. 3. The sensory papillae were slightly affected, but destruction of tegumental spine was not recognized. 4. The effect of praziquantel on the worm was found dependent on the concentration and incubation time, however, the effect was more dependent upon the incubation time. |
| Key Words:
Heterophyopsis continua, heterophyidae, praziquantel, perch, SEM |
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