| Distribution of T and B lymphocytes in peripheral blood and lymphoid tissues of bovine |
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Chang-yong Yoon, Tae-Joong Kim, Hyo-seok Chai, Jong-Myeog Kim, Hee-jong Song |
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College of Veterinary Medicine, Chonbuk National University |
| 소의 순환혈액 및 림프조직내 T 및 B 림프구 분포 |
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윤창용, 김태중, 채효석, 김종면, 송희종 |
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전북대학교 수의과대학 |
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| Abstract |
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This study was undertaken to identify the distribution of T and B lymphocytes in bovine peripheral blood and various lymphoid tissues by the method of ABPC using RABTS, $BLT_1$ and $_6E_{12}$ as primary antibodies. RABTS, $BLT_1$ and $_6E_{12}$ positive cells in PB-MNCs were 70.9${pm}5.5%$, $59.0{pm}8.7%$ and $23.0{pm}8.7%$, respectively. $BLT_1$ and $_6E_{12}$ positive cells in nylon wool nonadherent cells of PB-MNCs were $91.6{pm}1.0%$ and $9.6{pm}0.8%$, respectively. In the lymphoid tissues such as inguinal lymph node, mesenteric lymph node, spleen and thymus, positive cells of RABTS were $76.3{pm}3.4%$, $74.2{pm}8.2%$, $73.6{pm}5.5%$ and $95.6{pm}2.8%$, those of $BLT_1$ were $56.4{pm}6.2%$, $55.6{pm}7.7%$, $48.6{pm}5.1%$ and $23.0{pm}4.8%$ and those of $_6E_{12}$ were $45.3{pm}7.4%$, $42.3{pm}5.8%$, $48.5{pm}6.2%$ and $5.6{pm}2.1%$, in order. These results are indicating that nylon wool column method is effective for separation of bovine ocytes. |
| Key Words:
monoclonal antibodies, avidin-biotin peroxidase stain, mononuclear cells, peripheral blood, lymphoid tissues, Korean cattle |
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