Biological and physicochemical properties of canine parvovirus isolated from the dogs with diarrhea

Choi, Hae-yeon; Jun, Moo-hyung; Park, Seong-kuk

Korean Journal of Veterinary Research 1991;31(3):295-302.

Hae-yeon Choi¹, Moo-hyung Jun², Seong-kuk Park²

¹Northern Branch of Chungbuk Animal Health Laboratory
²College of Veterinary Medicine, Chungnam National University

설사증 이환견(罹患犬)으로 부터 분리한 canine parvovirus의 성상에 관한 연구

최해연¹, 전무형², 박성국²

¹충북 가축위생시험소 북부지소
²충남대학교 수의과대학

Abstract

From 1988 to 1989, 8 strains of canine parvovirus-2(CPV-2) were isolated from the fecal specimens from the dogs that were clinically diagnosed as canine parvoviral enteritis in the veterinary hospitals located in the regions of Taejeon and Chungbuk province. The biological and physicochemical properties for the isolates were studied. Among 62 fecal samples collected from the dogs with enteric diseases, 24(38.7%) showed the haemagglutinating activity to porcine erythrocyte ranging from 16 to 16,384 of HA titers. In cytopathological studies with CRFK cells, intranuclear inclusion bodies were observed in all of eight specimens with the high HA titer over 1,000, of which three specimens showed cytoplasmic inclusions concurrently with the intranuclear inclusion bodies. It was found that the isolates revealed the highest haemagglutinating activity with porcine erythrocytes and the relatively lower haemagglutination titers with the erythrocytes from cat and rabbit. None of erythrocytes from the other animals reacted with the isolates. By the cross-haemagglutination inhibition test for the isolates with the reference viruses and sera, the isolates were evidently identified as the strains of CPV-2. In physicochemical property test, the isolates were stable in lipid solvent, pH and heat treatment at $56^{circ}C$ for 30 min, and showed the virus particle size less than 25 nm, containing a DNA genome.

Key Words: Canine parvovirus, Korean isolates, biological and physicochemical properties