Detection of antibodies against infectious Borna disease virus -a comparison of three serological methods-

Lee, Du-sik

Korean Journal of Veterinary Research 1992;32(1):57-61.

Du-sik Lee

Department of Veterinary Medicine, Cheju National University

보르나병 바이러스 항체검출을 위한 연구 -세 가지 혈청진단법의 비교-

이두식

제주대학교 수의학과

Abstract

To determin the accuracy of serological methods in detecting Borna-disease(BD) viral antibodies, 273 experimentally infected rabbit sera were compared by using indirect immunofluorescence antibody test(IFA), serum neutralization test(SN) and enzyme-linked immunosorbent assay(ELISA). One hundred twenty-three serum samples had BD viral antibodies detected by IFA. CELISA antibodies to BD virus were also present in the same one hundred twenty-three serum samples. However, neutralization test antibodies to BD virus were present in 27 of the in rabbit serum samples. Neutralization test was sensitive in comparison with KFA and CELISA. In comparison with IFA, CELISA was both sensitive and specific in detecting BD viral antibodies. These results extend observations made with laboratory animals to the diagnosis of naturally infected animals.

Key Words: Borna disease virus, Indirect immunofluoresoence antibody test, Cell enzyme-linked immunosorbent assay, Serum neutralization test