Immunocytochemical identification of Mycobacterium bovis in tissues

Kim, Soon-bok; Sur, Jung-hyang; Moon, Oun-gyeong

Korean Journal of Veterinary Research 1993;33(1):119-123.

Soon-bok Kim¹, Jung-hyang Sur¹, Oun-gyeong Moon²

¹CoIlege of Veterinary Medicine, Gyeongsang National University
²Gyeongnam Provincial Institute of Animal Health

소 결핵균의 면역세포화학적 동정

김순복¹, 서정향¹, 문운경²

¹경상대학교 수의과대학
²경상남도 가축위생시험소

Abstract

The present study was intended to use the avidin-biotin-peroxidase-antiperoxidase complex (ABPAP) method for the identification of Mycobacterium bovis in the tissue sections of infected cattle. Antibodies and linksera for ABPAP procedure used in incubated order were rabbit anti-Mycobacterium polyvalent antibodies, goat anti-rabbit IgG, rabbit peroxidase-antiperoxidase complex, biotinyl-horse anti-rabbit IgG, and avidin-biotin-peroxidase complex. Where the bacterial antigen was localized by ABPAP, a dark brown deposit occurred in the cytoplasms of macrophages and Langerhans' giant cells of the granulomatous lesions. The method approved to be highly specific for the identification of the bacteria and allowed a precise localization of the bacterial antigen in infected cells.

Key Words: Immunocytochemical identification, mycobacterial antigen