| Prevalence of toxoplasma antibody in swine by microplate latex agglutination test in Kyeongnam district |
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Byung-hoon Lee1, Cha-yong Kim1, Myung-deuk Suh2 |
1Central branch of Kyeongnam Veterinary Service Laboratory
2College of Veterinary Medicine, Gyeongsang National University |
| Latex 응집반응에 의한 경남중부지역 돼지의 톡소플라즈마병 항체조사 |
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이병훈1, 김차용1, 서명득2 |
1경상남도 가축위생시험소 중부지소
2경상대학교 수의과대학 |
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| Abstract |
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This study was conducted to detect the serum antibody of toxoplasma in swine from breeding-pig, rearing-pig farm and slaughtered pig in abattior by latex agglutination(LA) test. The perfomance of LA test was carried out with commercial Toxo-MT kit(Eiken Chemical Co.)by Tsubota and Ozawa's method. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum titer of 1 : 32. Positive rate of toxoplasma antibody from the total of 823 serum samples by LA test was 17.0%(140 cases). And positive rates of toxoplasma antibody against serum samples of 194 from breeding-pig farm, 273 from rearing-pig farm and 356 from abattior were 91 cases(46. 9%), 23 cases(8.4%) and 26 cases(7.3%), respectively. The distributions of serum antibody titers in 823 test sera by LA test were shown 51 cases(36.3%) in 1:32, 40(28.6%) in 1:64, 17(12.1%) in 1:128, 14(10.0%) in 1:256, 3(2.1%) in 1:512, 5(3.6%) in 1:1024 and 3(2.1%) in 1:2048. The ranges of positive rate from the sera in each group of breeding-pig farms were 20~61.9%. |
| Key Words:
LA test, swine toxoplasma, Toxo-MT |
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