Identification of Aujeszky's disease virus by in situ hybridization

Kim, Soon-bok; Sur, Jung-hang; Moon, Oun-kyung

Korean Journal of Veterinary Research 1994;34(2):327-333.

Soon-bok Kim¹, Jung-hang Sur¹, Oun-kyung Moon²

¹College of Veterinary Medicine, Gyeongsang National University
²Kyongnam Veterinary Service Laboratory

In situ hybridization 조직화학법을 이용한 오제스키병 바이러스 동정

김순복¹, 서정향¹, 문운경²

¹경상대학교 수의과대학
²경남가축위생시험소

Abstract

The purpose of this study was to establish a rapid diagnostic method detecting Aujeszky's disease virus (ADV) DNA in the cultured cell monolayers (PK-15) and tissue sections of ADV(NYJ-1-87)-infected rats and pigs by in situ hybridization(ISH). Detection of specific ADV-DNA in infected cells was conducted by radiolabeled ISH method using $^{32}P-labeled $ DNA probe (BamH1 7 fragment) which contains a 6.3 Kb ADV-DNA insert. Where ADV-DNA was detected by radiolabeled ISH, the deposition of black photographic grains occurred in the nuclei and the cytoplasms of ADV-infected cells. Positive hybridization signal was often observed in the spinal trigerminal nucleus of the pons, the nucleus of the trigerminal ganglion neuron and the epithelial cells of tonsillar crypts. The results suggested that ISH is considered as a highly sensitive and reliable tool for confirmative diagnosis of this viral disease.

Key Words: radiolabeled in situ hybridization, Aujeszky's disease virus