Serological cross-reaction with Brucella abortus antigen extracted by sodium dodecyl sulfate and Yersinia enterocolitica 0:9

Lim, Yoon-kyu; Yang, Ki-chun; Lee, Kyung-kap; Park, Jun-hong; Lee, Du-sik; Park, Yong-ho; Kang, Seung-won; Mok, Ji-won; Lee, Yong-soon

Korean Journal of Veterinary Research 1995;35(1):143-148.

Yoon-kyu Lim¹, Ki-chun Yang¹, Kyung-kap Lee¹, Jun-hong Park¹, Du-sik Lee¹, Yong-ho Park², Seung-won Kang², Ji-won Mok³, Yong-soon Lee⁴

¹Department of Veterinary Medicine, Cheju National University
²Veterinary Research Institute, RDA
³National Institute of Safety Research
⁴College of Veterinary Medicine, Seoul National University

SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구

임윤규¹, 양기천¹, 이경갑¹, 박전홍¹, 이두식¹, 박용호², 강승원², 목지원³, 이영순⁴

¹제주대학교 수의학과
²농촌진흥청 가축위생연구소
³국립보건안전연구원
⁴서울대학교 수의과대학

Abstract

Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.

Key Words: Brucella abortus, Yersinia enterocolitica 0:9, cross-reaction, SDS, Western blot, ELISA