Effect of Artemisia messes-schmidiana var viridis on lipid and histopathology for 1-naphthylisothiocyanate-induced intrahepatic cholestasis in rat

Kim, Kil-soo; Jeong, Young-gil; Kim, Moo-kang

Korean Journal of Veterinary Research 1995;35(3):489-496.

Kil-soo Kim¹, Young-gil Jeong², Moo-kang Kim²

¹Department of Laboratory Animal Research, Asan Institute for Life Sciences
²College of Veterinary Medicine, Chungnam National University

1-naphthylisothiocyanate에 기인된 랫드의 간내성 담즙분비 정지에 대한 인진호(Artemisia messes-schmidiana var viridis)의 지질 및 조직병리학적 영향

김길수¹, 정영길², 김무강²

¹아산생명과학연구소 실험동물연구실
²충남대학교 수의과대학

Abstract

Artemisia messes-schmidiana var viridis(Compositae) has been used for jaundice, hepatitis, diuretic and liver cirrhosis etc. 1-naphthylisothiocyanate(ANIT) has been used as a model compound to study mechanisms of intrahepatic cholestasis in laboratory animals as rat and mouse. The purposes of present study are to examine pharmacological effects of Artemisia messes-schmidiana var viridis water extract(AMWE) on alterations of triacylglycerol, cholesterol, protein, albumin and A/G ratio levels in serum, of histopathological appearances of liver, and that of hepatic microsomal cytochrome P-450 contents. Increased serum triacylglycerol levels by ANIT were significantly decreased with AMWE. However, AMWE posttreatment aggravated ANIT-induced cholesterol increase. Serum total protein and albumin contents, and A/G ratio were decreased in all ANIT-treated groups, and there were increased compared with control by AMWE posttreatment. Hepatic microsomal cytochrome P-450 contents were decreased in either AMWE and ANIT treatment, which greatly increased with AMWE pretreatment. On the other hand, in histological findings, our results shown that ANIT induced increase of lipid droplets and widening of sinusoidal capillary and these phenomena were disappeared with AMWE treatment. In conclusion, AMWE have choleresis effect. Also, AMWE improved lipid metabolism, protection and regeneration of hepatocytes in ANIT-induced cholestasis.

Key Words: Artemisia messes-schmidiana var viridis, choleresis, ANIT, cholestasis, hepatoprotective effect