Production of monoclonal antibody(MAb) for lymphocytes of Korean native cattle 1. MAb to T lymphocytes

Song, Hee-jong; Woon, Jae-ho; Kim, Tae-joong; Chai, Hyo-seok; Hur, Boo-hong; Choi, Min-soon

Korean Journal of Veterinary Research 1995;35(4):769-776.

Hee-jong Song¹, Jae-ho Woon¹, Tae-joong Kim¹, Hyo-seok Chai², Boo-hong Hur², Min-soon Choi³

¹College of Veterinary Medicine, Chonbuk National Universtiy
²Chonbuk Veterinary Service Laboratory
³College of Ocean Science and Technology, Kunsan National University

한우 림프구에 대한 단일항체 생산 1. T 림프구에 대한 단일항체

송희종¹, 운재호¹, 김태중¹, 채효석², 허부홍², 최민순³

¹전북대학교 수의과대학
²전라북도 가축위생시험소
³군산대학교 해양산업대학

Abstract

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

Key Words: monoclonal antibody, T lymphocytes, Korean cattle, ELISA, immunoperoxidase