Detection of Theileria sergenti schizonts in lymphoid cell of lymph node in Korean cattle

Lee, Seung-ok; Lee, Joo-mook; Kwon, Oh-deog; Park, Jin-ho; Park, Sang-youl

Korean Journal of Veterinary Research 1997;37(1):189-194.

Seung-ok Lee, Joo-mook Lee, Oh-deog Kwon, Jin-ho Park, Sang-youl Park

College of Veterinary Medicine, Chonbuk National University

한우의 림프절내 림프구양 세포에서 Theileria sergenti schizonts의 검출

이승옥, 이주묵, 권오덕, 박진호, 박상열

전북대학교 수의과대학

Abstract

The life cycle of Theileria sergenti(T sergenti) in cattle, especially Korean native cattle, was not proved clearly. To find schizont stage in the life cycle of T sergenti in Korean cattle, T sergenti schizonts in the cells of parotid lymph nodes from 10 adult Korean cattle were examined. Lymphoid cells which were separated from these lymph nodes were cytocentrifuged to observe the parasites in the cells. T sergenti schizonts were detected in the cells of lymph nodes of 6 cattle out of them by IFA(Indirect Fluorescent Antibody) test and Giemsa stain. By peroxidase stain, the cells which contain schizonts were proved lymphoid cells. T sergenti schizonts identified by IFA test were able to be restained by Giemsa stain. Also, merozoites were observed in peripheral blood of the same 6 cattle that had schizonts, by giemsa stain, but not observed in the 4 cattle that had not been detected schizonts. As a part of life cycle of T sergenti, schizonts were observed in the lymphoid cells of Korean cattle.

Key Words: Theileria sergenti, schizont, lymph node, Korean native cattle