| The morphological and immunohistochemical changes of endocrine pancreas by pancreatic duct ligation in chicken |
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Sae-kwang Ku1, Jae-hyun Lee1, Hyeung-sik Lee2 |
1College of Veterinary Medicine, Kyungpook National University
2Department of Biology, Kyungsan University |
| 췌관을 결찰한 닭 췌장 내분비부의 형태학적 및 면역조직화학적 변화 |
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구세광1, 이재현1, 이형식2 |
1경북대학교 수의과대학
2경산대학교 생물학과 |
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| Abstract |
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To investigate morphological changes in the endocrine pancreas of chicken after pancreatic duct ligation, experimental animals were subdivided to control, 12 hours, 1 day, 2 days, 4 days, 7 days and 10 days groupes and all of three pancreatic ducts of chicken were ligated by surgical procedure and then the morphological changes were observed. In pancreatic islets, the vacuolation and invasion of connective tissue were occurred in all experimental groups and dissociation of pancreatic islets was detected in 4 days after pancreatic duct ligation and hold out to 10 days. The peak of the morphological changes in pancreatic islets was detected in 4 days after pancreatic duct ligation. In the results of immunohistochemical methods against glucagon, insulin, somatostatin and bovine pancreatic polypeptide(BPP), the number of immunoreactive pancreatic islets were decreased but the size increased with time, so the number of immunoreactive cells in each pancreatic islets were increased. Glucagon-immunoreactive cells were not changed but insulin-immunoreactive cells were decreased with time(p<0.05). BPP-immunoreactive cells were increased in 2 days after pancreatic duct ligation and then decreased with time(p<0.05). Somatostatin-immunoreactive cells were increased with time(p<0.05) in dark islets. |
| Key Words:
pancreatic duct ligation, chicken, immunohistochemical method, pancreatic islet |
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