| Immunogenicity of outer membrane protein extracted from Salmonella gallinarum in chickens |
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Hee-soo Lee1, Soon-jae Kim2, Ki-seuk Kim1, In-pil Mo1, Yong-ku Woo1, Yong-kuk Kwon1, Tae-jong Kim2 |
1National Veterninary Research Institute, RDA
2Department of Veterninary Medicine, Kon-Kuk University |
| Salmonella gallinarum 분리주로부터 추출한 세포외막 단백질의 닭에 대한 면역원성 |
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이희수1, 김수재2, 김기석1, 모인필1, 우용구1, 권용국1, 김태종2 |
1농촌진흥청 수의과학연구소
2건국대학교 수의학과 |
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| Abstract |
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Fowl typhoid caused by Salmonella gallinarum has increased dramatically since 1992 and has caused a great economic losses in chicken industry by characterizing with high mortality. In these studies, we investigated the immunogenicity and protectivity in chickens which were immunized with outer membrane protein(OMP) extracted from isolates of S gallinarum against challenge with live microorganism. Outer membrane proteins were composed of various sizes of molecular weight including 14K, 22K, 31K, 36K, 40K and 55K and the most of them responded strongly against rabbit antisera in immunoblot analysis. The chickens vaccinated with OMP or vaccinated with whole-cell combined with OMP($200{mu}g$/chickens) complex showed higher delayed type hypersensitivity(DTH) response than that of whole-cell vaccinated group. The protective rates of OMP or whole-cell combined with OMP complex group against challenge of S gallinarum were higher (above 75%) than those (45~50%) of whole-cell vaccinated group. All vaccines were safe and the body weight-gains of all vaccinated groups were not significantly different (p<0.05) from those of nonvaccinated control group. In vitro tests, OMP stimulated both the proliferation of lymphocytes and T-lymphocytes, and OMP-induced lymphocyte proliferation was higher in the cells of the immunized chickens with OMP than in those from the control chickens. |
| Key Words:
Salmonella gallinarum, outer membrane protein, immunogenicity |
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