| Immunogenetic characterization of Korean native chickens by monoclonal anibodies to chicken leukocyte differentiation antigens |
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Yong-ho Park1, Jae-yong Hahn2, Bong-kuk Oh2, Jin-san Moon3, Bok-kyeong Koo3, Yi-seok Joo3, Keun-seok Seo1, Hyun S Lillehoj4, William C Davis5 |
1College of Veterinary Medicine, Seoul National University
2College of Agricultural Life Science, Seoul National University
3National Veterinary Research Institute
4USDA-ARS
5Washington State University USA |
| 닭 백혈구 특이 단트론항체를 이용한 한국 재래닭의 유전 면역학적 특성에 관한 연구 |
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박용호1, 한재용2, 오봉국2, 문진산3, 구복경3, 주이석3, 서근석1, 4, 5 |
1서울대학교 수의과대학
2서울대학교 농업생명과학대학
3수의과학연구소
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5 |
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| Abstract |
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The immunogenetic analysis was performed to characterize the Korean native chickens (KNC) determined by monoclonal antibodies specific to chicken leukocyte differentiation antigens and flow cytometry. A total of 174 chickens including 58 KNC (black, brown and darkbrown colored), 77 foreign breed (Nagoya, White Reghorn, Rhode Island and Cornish) and 39 mixed breed (19 KNC with Nagoya and 20 KNC with Rhode Island) separately growing at Animal Science and Technology Institute were examined. The proportion of cells expressing MHC class II molecule (B-L in chicken) was significantly high in KNC. Proportion of CD4+ T helper cells was also higher in KNC and two mixed breed than that in foreign breed. However, proportion of CD8+ cells and TCR1 + (${gamma}^{delta}$ T cell receptor) cells was the lowest among the breed examined. Otherwise, those proportions were significantly high in White leghorn and two mixed breeds with two exclusive subpopulations. The two subpopulations were also typically shown in MHC class $II^+$ cells in KNC and one mixed breed, black-colored KNC with Nagoya. Although genotypic analysis was not pursued to characterize the immunogenetic properties of KNC, difference of phenotypic expression based on leukocyte differentiation molecules could be elucidated in KNC in this study. |
| Key Words:
flow cytometry, MHC class II, TCR1, CD4+ T cell, CD8+ T cells |
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