Seroepidemiological study on porcine cytomegalovirus to pigs in Korea

Kang, Mun-il; Han, Mi; Tajima, Tomoko; Han, Dong-un; Kim, Hee-sun; Kim, Byung-han; Kim, Hong-jib; Ahn, Soo-hwan

Korean Journal of Veterinary Research 1998;38(4):756-762.

Mun-il Kang¹, Mi Han¹, Tomoko Tajima², Dong-un Han¹, Hee-sun Kim¹, Byung-han Kim³, Hong-jib Kim⁴, Soo-hwan Ahn³

¹College of Veterinary Medicine, Chonnam National University
²Department of veterinary Science, Osak Prefecture University
³Department of Virology, National Veterinary Research and Qurantine Service
⁴Institute of Animal Science, Daesang Co, Ltd

국내 사육 돈군내 Porcine cytomegalovirus에 관한 혈청역학적 연구

강문일¹, 한미¹, ², 한동운¹, 김희선¹, 김병한³, 김홍집⁴, 안수환³

¹전남대학교 수의과대학
²일본대판부계시학원농학부수의학과
³국립수의과학검역원 바이러스과
⁴대상(주) 축산과학연구소

Abstract

The purpose of this study was to investigate the confirmation and prevalence of porcine cytomegalovirus (PCMV) infection of pigs in Korea using enzyme-linked immunosorbent assay (ELISA). Four hundred-eighty one sera tested were collected from the areas of Kyonggi, Kangwon, Chungcheong, Cholla, Gyongsang and Cheju during the year of 1991 to 1997 except 1994. PCMV antigen, OF-1 strain, for ELISA, was prepared 19-PFT-F cell line originated from porcine fallopian tube. Positive control was used by sera made from the specific pathogen free piglets which were infected with OF-1 strain. Three hundred-sixty seven of 481 sera (76.3%) were positive against PCMV. Antibody titers of these seropositives were classified by 129 (26.8%) cases in more than 1 : 12,800, 77 (16.0%) in 1 : 6,400, 76 (15.8%) in 1 : 3,200, 44 (9.2%) in 1 : 1,600, and 41 (8.5%) in 1 : 800, respectively. Also, the seropositive pigs were divided by 87.4% (76/87) in older than 6 month-old, 73.9% (238/322) in 2~6-month old, and 73.6% (53/72) in less than 2-month old, respectively. Regional prevalence rate of PCMV infection in Korea showed 89.7% (70/78) in Chungchong, 79.8/% (83/104) in Cholla, 79.4% (143/180) in Kyonggi, 79.3% (42/53) in Gyongsang, 50% (15/30) in Kangwon, and 38.9% (14/36) in Cheju area, respectively. In the sera collected in 1991, seropositive rate was appeared as 90.2% (37/41). From 1992 to 1997 except 1994, the average infection rate to PCMV was 77.5%. Consequently, these results confirmed that PCMV in Korean piggeries was introduced at least before the year of 1991. More importantly, PCMV infection has been prevailing nation-wide in pig herds in Korea.

Key Words: porcine cytomegalovirus(PCMV), ELISA, inclusion body rhinitis, serology