Antigenic-types of Salmonella pullorum and Salmonella gallinarum isolated from poultry in Korea

Woo, Yong-ku; Kim, Bong-hwan

Korean Journal of Veterinary Research 1998;38(4):777-783.

Yong-ku Woo¹, Bong-hwan Kim²

¹National veterinary Research and Quarantine Services
²College of Veterinary medicine, Kyungpook National University

우리나라의 닭에서 분리한 Salmonella pullorum과 Salmonella gallinarum의 항원형

우용구¹, 김봉환²

¹농림부 수의과학검역원
²경북대학교 수의과대학

Abstract

Antigenic types of 114 Salmonella pullorum and 152 Salmonella gallinarum field isolates were evaluated. All 3 antigenic types were identified among field isolates of S pullorum by factor-serum analysis but the majority of them were standard type(90.4%). Of the 114 S pullorum isolates, only eight(7.0%) were intermediate type and 3(2.6%) were variant type. Using the ammonium sulfate precipitation(ASP) test, one-hundred and three(90.4%) S pullorum isolates were standard type, while intermediate and variant types were 8.4% and 1.4%, respectively. One-hundred and fifty-two S gallinarum isolates were identified as standard type by ASP test and serological analysis. According to the random amplified polymorphisms of DNA(RAPD) patterns, most of S pullorum isolates were differentiated with 3 types in their fragment-patterns. No correlations were found between SDS-PAGE profiles and antigenic types of S pullorum isolates.

Key Words: S pullorum, S gallinarum, antigenic types, factor serum, ASP, RAPD