| Diagnosis of bovine tuberculosis by single intradermal comparative tuberculin test and gamma-interferon assay |
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Yun-sang Cho1, Jong-man Kim1, Suk-chan Jung1, Seung-ryong Woo1, Jong-yeom Kim1, Han-sang Yoo2, Yong-ho Park2, Jong-sam Ahn1 |
1National Veterinary Research and Quarantine Service
2College of Veterinary Medicine, Seoul National University |
| 비교 피내 검사와 감마 인터페론 검사에 의한 우결핵의 진단 |
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조윤상1, 김종만1, 정석찬1, 우승룡1, 김종염1, 유한상2, 박용호2, 안종삼1 |
1국립수의과학검역원
2서울대학교 수의과대학 |
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| Abstract |
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Since Robert Koch found tubercle bacilli in 1882, the studies on tubercle bacilli of human and animal had been carried out. Being old tuberculin(OT) introduced in 1890, the specificity of the diagnosis of tuberculosis has been improved by continual uses of heat concentrated synthetic medium(HCSM) and purified protein derivatives(PPD) tuberculin. Now, two types of tuberculin test are used worldwidly ; the single intradermal test(SIT) using bovine tuberculin and the single intradermal comparative tuberculin test(SICTT) using avian and bovine tuberculins. In the SICTT, each countries have used with different combination of both avian and bovine tuberculins' titers. However, this kinds of studies have not reported in Korea. Therefore, the studies on the combination of their tuberculins' titers were performed through intradermal test of guinea pigs sensitized with either Mycobacterium bovis or M avium and were examined in 10 cattles of SIT positive reactors. Also, IFN-${gamma}$ assay, the latest diagnostic method of bovine tuberculosis, was experimentally applied to SIT positive reactors. For determining the optimal titers, sensitized guinea pigs with M bovis and M avium were intradermally injected avian and bovine tuberculin. In guinea pigs sensitized with M bovis, bovine tuberculin 50 T.U. showed significant difference from all tested concentrations of avian tuberculin(p < 0.05). In guinea pigs sensitized with M avium, there is significantly different between bovine tuberculin and avian tuberculin by 25 T.U.(p < 0.01). Therefore, optimal titers of bovine and avian PPD tuberculins' titers for the SICTT in Korea were 5,000 and 2,500 tuberculin units, respectively, and the swelling diffences between bovine and avian site in SIT positive reactors were above 3mm. Also, in IFN-${gamma}$ assay, the 9 SIT positive reactors were showed all the positive reactions. |
| Key Words:
single intradermal comparative tuberculin test, IFN-${gamma}$ assay |
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