Sequential pathologic change of Korean Neospora caninum isolates in mice

Bae, Ji-seon; Kim, Jae-hoon; Lee, Jung-keun; Lee, Byung-chun; Choi, Yang-kyu; Hyun, Byung-hwa; Kim, Dae-yong

Korean Journal of Veterinary Research 2000;40(1):145-152.

Ji-seon Bae¹, Jae-hoon Kim², Jung-keun Lee¹, Byung-chun Lee¹, Yang-kyu Choi³, Byung-hwa Hyun³, Dae-yong Kim¹

¹College of Veterinary Medicine, Seoul National University
²National Veterinary Research and Quarantine Service
³Korea Research Institute of Bioscience and Biotechnology

Neospora caninum 국내 분리주의 경시적 변화

배진선¹, 김재훈², 이중근¹, 이병천¹, 최양규³, 현병화³, 김대용¹

¹서울대학교 수의과대학
²국립수의과학검역원
³생명공학연구소

Abstract

This study was performed to study the sequential clinical and pathologic changes of Korean isolate KBA-2 of Neospora caninum in SCID mice following intraperitoneal infection. Also the results of PCR and in vitro isolation was compared during the study. The infection appears to be disseminated hematogenously, when the infection was chased every 3days up to 21 days following infection. The PCR method was determined to be more effective than in vitro isolation regarding early detection of the organism follwing infection.

Key Words: Neospora caninum, PCR, isolation, dissemination, pathogenesis