| In vivo characterization and transmission of Korean foot-and-mouth disease virus(FMDV) |
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Jung-hyang Sur1, Jin-ho Shin2, Juan Loubroth1, Max Yeh1, Bok-kyung Ku2, Kang-seuk Choi2, Byung-joon Kweon2, Hyun-joo Sohn2, Young-joon Ko2, Cheong-up Choi2, Chang-hee Kwon2, Jong-yeom Kim2, Soo-hwan An2, Ki-seuk Kim2, Oun-kyung Moon2, Jae-hoon Kim2, Sang-ho Choi2, Hong-gil Lee2, Eui-kyung Hwang2, Soon-bok Kim3, Shin-seuk Kang4, Ok-kyung Kim2 |
1Plum Island Animal Disease Center, ARS, United Sates Deparement of Agriculture
2National Veterinary Research Quarantine Service
3College of Veterinary Medicine, Gyeongsang National University
4Chungbuk Veterinary Service Laboratory |
| 국내 발생 구제역 바이러스(foot-and-mouth disease virus)의 특성과 전파력에 관한 연구 |
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서정향1, 신진호2, 1, 1, 구복경2, 최강석2, 권병준2, 손현주2, 고영준2, 최정업2, 권창희2, 김종염2, 안수환2, 김기석2, 문운경2, 김재훈2, 최상호2, 이홍길2, 황의경2, 김순복3, 강신석4, 김옥경2 |
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2국립수의과학검역원
3경상대학교 수의과대학
4충북축산위생연구소 |
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| Abstract |
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A study was conducted to determine the susceptibility of swine to Korean foot-and-mouth disease virus (FMDV; subtype O, isolated from Chungju province) in April, 2ooo. One holstein cow was inoculated intradermolingually with suspension of homogenized tissue from a Korean native cow naturally infected with Korean FMDY. Infected cow was housed with one susceptible cow and one susceptible pig (contact sentinels). Four additional susceptible pigs were housed in the same room but caged separately (non-contacted sentinels). The contacted pig and cow as well as non-contact pigs developed typical clinical signs after 2, 3, and 7 days post exposure, respectively. We compared neutralizing antibody from the animals to FMDV $O_1$ Lombardy, O Taiwan, $O_1$ Campos, and $O_1$ Manisa after 0, 4, 7, 10, 14, 21, 28 days post challenge and post-exposure. The highest viral neutralization titer could be interpreted that serotype O Korea (Chungju isolate) is antigenically more related to $O_1$ Manisa. In addition, immunohistochemistry was used to further characterize Korean FMDV from tissues of infected pigs. Korean FMDV antigen was observed in the tongue, hoof, esophagus, and tonsil tissues of sentinel pigs. These findings suggest that Korean FMD virus isolated from cattle can be rapidly transmitted to pigs both directly and indirectly contrast field observation in which only cattle were clinically ill. |
| Key Words:
Korean foot-and-mouth-disease, Chungju isolate transmission, immunohistochemistry, viral neutralization |
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