An immunohistochemical study of the serotonin-immunoreactive cells in the developing pancreas of the chicken embryos

Ham, The-su

Korean Journal of Veterinary Research 2001;41(2):133-138.

The-su Ham

College of Natural Resources, Yeungnam University

발생단계에 따른 닭 태자 췌장에서 serotonin 면역반응세포에 대한 면역조직화학적 연구

함태수

영남대학교 자연자원대학 생물자원학부

Abstract

The distributions and relative frequencies of the serotonin-immunoreactive cells were studied in dorsal, ventral, third and splenic lobes of developing chicken pancreas during embryonic periods (10 days of incubation to hatching) by immunohistochemical methods. The regions of pancreas were subdivided into three regions, exocrine, light and dark islets. Round and/or oval shaped serotonin-immunoreactive cells were detected in all four lobes. According to developmental stages, the types of lobes and the regions of pancreas, these immunoreactive cells were showed various distributions and relative frequencies. In exocrine portions, serotonin-immunoreactive cells were found in the splenic lobes at 13-14 days of incubation, in the third lobes from 10 days to 19 days of incubation, in the ventral lobes from 10 days of incubation to hatching and in the dorsal lobes from 11 days of incubation to hatching. In pancreatic islets, these cells were detected only in the dark islets of splenic lobes at 15 and 16 day of incubation with rare frequency. In conclusion, serotonin-immunoreactive cells decreased with developmental stages in all four lobes and their relative frequencies decreased with developmental stages.

Key Words: Chicken embryo, developing pancreas, serotonin, immunohistochemistry