Analysis of antigenic sites on the VP4 of porcine rotavirus, Gottfried strain

Song, Yun-kyung; Kim, Won-yong; Kang, Shien-young

Korean Journal of Veterinary Research 2001;41(3):343-350.

Yun-kyung Song¹, Won-yong Kim², Shien-young Kang¹

¹Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University
²College of Medicine, Chungang University

돼지 로타바이러스(Gottfried 주)의 VP4 항원구조분석

송윤경¹, 김원용², 강신영¹

¹충북대학교 수의과대학/동물의학연구소
²중앙대학교 의과대학

Abstract

The neutralization epitopes of the outer capsid protein VP4 of a porcine rotavirus, Gottfried strain, were studied using neutralizing monocolonal antibodies(N-MAbs). Eight N-MAbs which are specific for the VP4 of Gottfried strain were used for analyzing the antigenic sites of VP4. Three different approaches were used for this analysis; i)testing the serological reactivity of each N-MAb against different G and P types of human and animal rotavirusese ii) analyzing N-MAb-resistant viral escape mutants and iii) performing nucleotide sequence analysis of the VP4 gene of each N-MAb-resistant viral escape mutant. From experimental results, at least four antigenic sites(I, II, III, and IV) were identified. Antigenic site I recognized by N-MAbs 24B9, 23G10, and 26A2 was separated from antigenic site II recognized by N-MAbs 30H5, 32B3, and 29B3. However, these antigenic sites were overlapped with antigenic site III recognized by N-MAb 21A1. The other antigenic site IV recognized by N-MAb 16D2 was separated from antigenic sites I, II, and III.

Key Words: Rotavirus, VP4, antigenic site, N-MAb resistant escape mutant, SSCP