| Comparison of Antigenic Spots between Escherichia coli O157:H7 Strains by 2-Dimensional Gel Electrophoresis |
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Yeong-Chang Ahn, Gee-Wook Shin, Yong-Seung Shin, Eung-Goo Lee, Hyoung-Jun Lee, Mi-Rim Park, Young-Rim Kim, Tae-Sung Jung, Gon-Sup Kim, Yong-Hwan Kim |
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Institute of Animal Medicine, College of Veterinary Medicine, Gyeong Sang Nation University |
| 이차원전기영동을 이용한 Escherichia coli O157:H7 균주간 항원 Spot의 비교 |
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안영창, 신기욱, 신용승, 이응구, 이형준, 박미림, 김영림, 정태성, 김곤섭, 김용환 |
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경상대학교 수의과대학 동물의학연구소 |
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| Abstract |
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Proteomics is an emerging powerful tool in studying protein expression and function. At present study, proteomics was employed to evaluate the antigenicity among Escherichia coli O157:H7 strains using 2-dimensional gel electrophoresis (2-DE) and immunoblotting, SDS-PAGE and immunblotting analysis revealed no big differences among E coli O157:H7 strains. 2-DE analysis, however, revealed common antigens as well as specific antigens. The immunoblotting analysis revealed 20 common antigenic spots among E coli O157:H7 strains. In addition, there were 3 and 13 spots as common antigens between ATCC 43894 and KSC 109, and between ATCC 43894 and ACH 5, respectively. Antigenic spots specific for individual strain were also identified as 15, 8 and 22 for ATCC 43894, ACH 5 and KSC 109, respectively. The common antigens would be useful by employing either vaccine development or diagnosis marker, or both, whereas the specific antigens of individual strains would be applicable for epidemiological study. This study suggest that proteome analysis, representative as 2-DE, is valuable tool in exploring the E. coli antigenicity. |
| Key Words:
E. coli, 2-DE, immunoblotting, antigenic spots |
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