Blood glucose change after surgical administration of insulin formula into rat intestinal regions

Kim, Nam Joong; Kim, Myung-Cheol

Korean Journal of Veterinary Research 2002;42(2):283-288.

Nam Joong Kim¹, Myung-Cheol Kim²

¹LGCI Life Science R&D, Animal Health Research
²College of Veterinary Medicine, Chungnam National University

Rat의 intestine 각 부위에 수술적으로 투여 된 insulin 제제에 의한 혈당 변화

김남중¹, 김명철²

¹LGCI 생명과학기술연구원 동물의약연구소
²충남대학교 수의과대학

Abstract

The present study was carried out to examine the effect of insulin formula on blood glucose change in normal Sprague-Dawley male rats. Also, this study was performed to investigate the feasibility of oral insulin formula development. To administrate the insulin formula into intestine, the surgical technique, celiotomy, was performed in rats. Insulin formula was administrated at a dose of 24.5 IU/kg via duodenum, ileum, and colon of the rats, and the blood glucose level was measured. For the comparison, the vehicle without insulin was administrated into ileum via celiotomy. Also, this insulin formula was administrated into rats orally using sonde and the same parameter was treasured. The bloods of all groups were collected from tail veins using syringes at given time interval. Orally administrated group did not show the change of blood glucose level and control group slightly show the change of blood glucose level at 1 hour after celiotomy. All intestinally administrated groups showed the change of blood glucose level. Among the tested groups, ileac administration group and colonic administration group showed the significant change of blood glucose level. Particularly, ileac administration group showed the lowest blood glucose level. To calculate the bioavailability of intestinal and oral administration, insulin solution was injected subcutaneosly, common insulin injection route, into another normal rats. The bioavailability of ileac group was 8.3% when compared with subcutaneous injection, duodenal group was 1.8%, colonic group was 4.2%, and oral group was 0.2%, respectively.

Key Words: insulin, blood glucose, intestinal absorption, celiotomy