Inadequacy of application of cytokinesis-blocked cells in fish (Rock fish, Sebastes schlegeli) and fowl(chicken) as biological dosimeter for radiation exposure |
Se-Ra Kim1, Tae-Hwan Kim2, Si-Yun Ryu3, Jong-Sik Jang4, Mi-Young An5, Sung-Ho Kim1 |
1College of Veterinary Medicine, Chonnam National University 2Korea Cancer Center Hospital 3College of Veterinary Medicine, Chungnam National University 4Department of Animal Science, Sangju National University 5College of Veterinary Medicine, Texas A&M University |
방사선 피폭의 생물학적 선량측정에 어류(조피볼락, Sebastes schlegeli) 및 조류(닭)의 세포질분열 차단 세포 적용의 부적절성 |
김세라1, 김태환2, 류시윤3, 장종식4, 안미영5, 김성호1 |
1전남대학교 수의과대학 2한국원자력병원 3충남대학교 수의과대학 4상주대학교 축산학과 5Texas A&M University 수의과대학 |
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Abstract |
The purpose of the present experiment was to investigate the micronuclei (MN) frequency in cytokinesis-blocked (CB) cells after various doses of gamma-rays in two species (fish and fowl) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors of two species. No binucleated cell was noted in erythrocyte. The peaks of binucleated lymphocyte formation were found at a concentration of 2% phytohaemagglutinin (PHA) and $3{mu}g/m{ell}$ cytochalasin B (Cyt-B) in fish at 144 hours after incubation and 2% PHA and $6{mu}g/m{ell}$ Cyt-B in fowl at 72 hours after incubation. But the micronucleus counts failed to show any evidence of radiation damage. Measurements performed after irradiation showed a dose-related decrease in the formation of binucleated cells in each of the donors studied. Results indicated that the assays were not suitable for this due to blastization inhibition (binucleation failure) after irradiation. We concluded that the use of CB cell from fish and fowl for detecting the results of mdiation exposure was highly questionable. |
Key Words:
Micronuclei, Fish, Fowl, Cytokinesis-blocked cell |
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