Establishment of Neospora agglutination test for serologic diagnosis of neosporosis

Kang, Min-Soo; Kim, Jae-Hoon; Hwang, Woo-Suk; Nam, Ho-Woo; Youn, Hee-Jeong; Bae, Jong-Hee; Kim, Dae-Yong

Korean Journal of Veterinary Research 2003;43(4):677-681.

Min-Soo Kang¹, Jae-Hoon Kim², Woo-Suk Hwang¹, Ho-Woo Nam³, Hee-Jeong Youn¹, Jong-Hee Bae², Dae-Yong Kim¹

¹College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
²Department of Veterinary Medicine, Cheju National University
³Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University

Neospora응집반응을 이용한 네오스포라증의 혈청학적 진단

강민수¹, 김재훈², 황우석¹, 남호우³, 윤희정¹, 배종희², 김대용¹

¹서울대학교 수의과대학
²제주대학교 수의학과
³카톨릭대학교 의과대학

Abstract

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

Key Words: Neospora caninum, Neospora agglutination test (NAT), serology, Korean dairy cattle, dog