Proteome analysis: Salmoenlla enteritidis antigen proteins

Park, Mi-rim; Shin, Yong-seung; Han, Dae-yong; Kim, Yong-hwan; Jung, Tae-sung; Lee, Hu-Jang; Lee, Eung-Goo; Kim, Jong-su; Kim, Eun-hee; Kim, Gon-Sup

Korean Journal of Veterinary Research 2004;44(1):57-64.

Mi-rim Park¹, Yong-seung Shin¹, Dae-yong Han¹, Yong-hwan Kim¹, Tae-sung Jung¹, Hu-Jang Lee¹, Eung-Goo Lee², Jong-su Kim¹, Eun-hee Kim³, Gon-Sup Kim¹

¹Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University
²SungGyunKwan University
³Jinju International University

Proteomics 기법을 이용한 Salmonella enteritidis의 항원 단백질 분석

박미림¹, 신용승¹, 한대용¹, 김용환¹, 정태성¹, 이후장¹, 이응구², 김종수¹, 김은희³, 김곤섭¹

¹경상대학교 수의과대학(생명과학연구소)
²성균관대학교
³진주국제대학교

Abstract

The common pathogen Salmonella enteirtidis (S. enteritidis) is the major cause of foodborne disease. Protein identification by peptide mass fingerprinting using the matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analysis unambiguously identity the spots from 2-dimensional electrophoresis (2-DE) gel. In this report, we examined protein components from patterns of S. enteritidis proteins. In addition, antigens that are recognized by sera can be identified by immunoblotting. This study that 2-DE analysis of S. enteritidis yields useful information concerning S. enteritidis proteome, the results that have been obtained led to a more detailed understanding of Salmonella pathology and open further interesting fields for future work.

Key Words: Salmonella enteirtidis, matrix-assisted laser desorption ionization time of fight, 2-dimensional electrophoresis, antigens, proteome