Proteome analysis of chickens fed with tissue culture medium waste after harvest of Korean wild ginseng

Seol, Jae-Won; Hwang, In Ho; Chae, Joon-Seok; Kang, Hyung-Sub; Ryu, Kyeong-Seon; Kang, Chun-Seong; Park, Sang-Youel

Korean Journal of Veterinary Research 2005;45(2):155-161.

Jae-Won Seol¹, In Ho Hwang², Joon-Seok Chae¹, Hyung-Sub Kang¹, Kyeong-Seon Ryu³, Chun-Seong Kang⁴, Sang-Youel Park¹

¹Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University
²National Livestock Research Institute
³Department of Animal Resources and Biotechnology, Chonbuk National University
⁴E&T Co. Ltd.

산삼 배양액을 급여한 육계에서 근육의 프로테옴 분석

설재원¹, 황인호², 채준석¹, 강형섭¹, 류경선³, 강춘성⁴, 박상열¹

¹전북대학교 수의과대학 생체안전성연구소
²축산기술연구소
³농업생명과학대학 동물자원과학부
⁴(주)이앤티

Abstract

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

Key Words: Proteomic analysis, Chicken, Gienseng, MALDI-TOF