| Application of a solid-phase fluorescence immunoassay to determine streptomycin residues in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major) |
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Hee Sik Chung1, Suk Kim2, Myung Sun Chun3, Won Chul Jung2, Dong Hyeok Kim2, Ho Yeong Shon4, Jeong Ju Lim2, Hu-Jang Lee2 |
1Hapcheon Country Office
2Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University
3College of Veterinary Medicine, Seoul National University
4Agricultural Technology Center, Yangsan City Hall |
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| Abstract |
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Parallux (IDEXX Laboratories, USA), a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of antibiotics in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Fishes were dipped in streptomycin 20 g/ton water, the recommended therapeutic dose, for 3 consecutive days. Muscle was sampled after 1st, 2nd, 3rd, 4th and 5th day drug treatment. The concentration of streptomycin in muscle, determined by SPFIA, was compared with that of internal standard (200 ppb as streptomycin). The absorbance ratio of sample to internal standard (S/C) was employed as an index to determine the muscle residues in fishes. The standard solutions were added to muscle samples to give final concentrations in muscle of 0.2 and 0.5 mg/ml to investigate the recovery rate. The recovery rates of all spiked samples were > 84% of the spiked value. Streptomycin was detected in muscles of fishes treated after the 1st day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting muscle tissue residues for streptomycin in farmed fishes. |
| Key Words:
olive flounder, red sea bream, rockfish, solid-phase fluorescence immunoassay, streptomycin |
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