In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets

Oh, Yu-Ri; Lee, Joong-Bok; Park, Seung-Yong; Song, Chang-Seon; Choi, In-Soo; Kim, Yong-Hyun; Han, Eun-Jung; Lee, Jung-Hee; Lim, Kwang-Sei; Huh, Chul-Sung; Kim, Seong-Hee; Park, Sang-Shin; Lee, Sang-Won

Korean Journal of Veterinary Research 2008;48(2):167-174.

Yu-Ri Oh¹, Joong-Bok Lee¹, Seung-Yong Park¹, Chang-Seon Song¹, In-Soo Choi¹, Yong-Hyun Kim², Eun-Jung Han³, Jung-Hee Lee³, Kwang-Sei Lim³, Chul-Sung Huh³, Seong-Hee Kim⁴, Sang-Shin Park⁵, Sang-Won Lee¹

¹College of Veterinary Medicine, Konkuk University
²Bio Medic Support
³R&D Center, Korea Yakult Co.
⁴National Veterinary Research & Quarantine Service
⁵Graduate School of Public Health, Seoul National University

Abstract

Lactic acid bacteria have been reported their beneficial roles on host including reduction of infectious diarrhea problems. In this study, preventive effect of Lactobacillus (L.) reuteri HY25101 and L. johnsonii HY25103 on porcine epidemic diarrhea virus (PEDV) was investigated in suckling piglets. Two groups of one day old PEDV naive piglets were orally administered L. reuteri HY25101 and L. johnsonii HY25103 for three days respectively before challenge with lethal dose of PEDV. In second experiment, passive immunized one day old piglets using colostrums containing PEDV specific IgA were used. The survival rates of the L. reuteri HY25101 administered group were significantly higher than that of L. johnsonii HY25103 administered group and viral shedding was rapidly diminished in L. reuteri HY25101 administered group. Interestingly piglets born from the sow immunized with attenuated PEDV vaccine were not completely protected from PEDV challenge, however coadministeration of L. reuteri HY25101 and colostrums containing PEDV specific IgA were more effectively prevent PEDV infection. These results suggested that dietary treatment using L. reuteri HY25101 could reduce diarrheal problem and mortality rate caused by PEDV in suckling pigs. In addition, L. reuteri HY25101 could be used as one of effective compensation treatment with attenuated live vaccine for PED.

Key Words: lactic acid bacteria, Lactobacillus johnsonii, Lactobacillus reuteri, porcine epidemic diarrhea, probiotic