| The polymorphism of Theileria buffeli major surface protein associate with their clinical signs in holstein in Korea |
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Do-Hyeon Yu1, Ying-Hua Li1, Joon-Seok Chae2, Jin-Ho Park1 |
1College of Veterinary Medicine & Biosafety Institute, Chonbuk National University
2College of Veterinary Medicine, Seoul National University |
| 국내 젖소에서 Theileria buffeli 주요 표면 단백질 유전자의 다양성 분석 |
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유도현1, 이영화1, 채준석2, 박진호1 |
1전북대학교 수의과대학
2서울대학교 수의과대학 |
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| Abstract |
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Theileria (T.) buffeli (formerly T. sergenti/T. orientalis) is the major hemo-protozoan distributed in the Far East Asian countries such as Korea, China and Japan. It is responsible for the clinical symptoms of anorexia, ateliosis, anemia, fever and icterus. It also causes abortion and sudden death under severe cases, resulting in economic losses for many livestock farms. The objective of this study was to analyze the genetic diversity of the major surface protein (Msp) gene in T. buffeli in Holstein in Korea, and we characterized the association of the diversification of the Msp gene and its relationship with the pathogenicity of Theileria. For this, complete blood counts and Theileria PCR sequence analysis were performed from 57 Holstein in Jeju Island. A total of 26 PCR positive Holstein (16 anemic and 10 non-anemic) were then randomly selected based on 18s rRNA sequence typing of the Theileria Msp gene. The DNA sequence of the T. buffeli Msp gene in Holstein showed 99.0%, 99.2%, 99.9%, 99.5%, 98.7%, 98.4% and 98.4% homology with T. sergenti, Theileria spp., T. sergenti, Theileria spp., Theileria spp., Theileria spp. and Theileria spp., respectively. The result showed a genetic variation of 57.7% (type I), 3.8% (type II), 15.4% (type III), 7.7% (type IV), 13.5% (type V) and 1.9% (type VI). Type I is the most frequent type in both anemic and non-anemic Holstein while type II was found in only non-anemic Holstein. This results of our study help confirm the diversity of Msp gene types and demonstrate that the gene type distribution of Msp genes varies among Theileria-infected Holstein in Jeju Island. |
| Key Words:
anemia, Antigenic diversity, Korean indigenous cattle, Major surface protein gene, Theileria buffeli (sergenti/orientalis) |
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