An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis

Barate, Abhijit K.; Lee, Hwi-Young; Jeong, Hye-Won; Truong, Lam Quang; Joo, Hong-Gu; Hahn, Tae-Wook

doi:2012.52.1.39

Korean J Vet Res > Volume 52(1); 2012 > Article

Original Article

Korean Journal of Veterinary Research 2012;52(1):39-43.
DOI: https://doi.org/10.14405/kjvr.2012.52.1.039 Published online March 31, 2012.

An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis

Abhijit K. Barate¹, Hwi-Young Lee¹, Hye-Won Jeong¹, Lam Quang Truong¹, Hong-Gu Joo², Tae-Wook Hahn¹

¹Department of Veterinary Medicine, College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University
²Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University

Abstract

A multiplex PCR was developed for the simultaneous detection and differentiation of Mycoplasma (M.) hyopneumoniae and M. hyorhinis in clinical samples. Improved sensitivity is advantage of this technique over the previously reported multiplex assay. It was capable of detecting as little as 125 fg genomic DNA from M. hyopneumoniae and 62.5 fg genomic DNA from M. hyorhinis. Application of this multiplex PCR method to field isolates showed that M. hyopneumoniae and M. hyorhinis were present in 29% (107 of 370) of lung specimens and no mycoplasmas were detected in 56% (208 of 370) of the slaughtered pigs' lungs. At the farm level, M. hyopneumoniae and M. hyorhinis were detected in 34 of 36 (94.4%) randomly selected farms. We conclude that this assay would prove itself a value tool for monitoring these mycoplasmal infections and both M. hyopneumoniae and M. hyorhinis have been widely spread in swine herds of Korea.

Key Words: enzootic swine pneumonia, multiplex PCR, Mycoplasma