Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

Kim, Yong Kwan; Kim, Ha-Young; Jeon, Albert Byungyun; Lee, Myoung-Heon; Bae, You-Chan; Byun, Jae-Won

doi:2014.54.2.113

Korean J Vet Res > Volume 54(2); 2014 > Article

Brief Communication

Korean Journal of Veterinary Research 2014;54(2):113-115.
DOI: https://doi.org/10.14405/kjvr.2014.54.2.113 Published online June 30, 2014.

Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

Yong Kwan Kim, Ha-Young Kim, Albert Byungyun Jeon, Myoung-Heon Lee, You-Chan Bae, Jae-Won Byun

Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency

Abstract

Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were $10^4$, $10^5$ and $10^2$ CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.

Key Words: detection limit, invA, loop-mediated isothermal amplification assay, Salmonella