Isolation and molecular identification of Vibrio harveyi from the look down fish (Selene vomer)

Jun, Jin-Woo; Lee, Young-Ran; Giri, Sib Sankar; Kim, Hyoun-Joong; Yun, Sae-Kil; Chi, Cheng; Kim, Sang-Guen; Koh, Jeong-Rack; Jung, Ji-Yun; Lee, Byeong-Chun; Park, Se-Chang

doi:2016.56.2.125

Korean J Vet Res > Volume 56(2); 2016 > Article

Case report

Korean Journal of Veterinary Research 2016;56(2):125-127.
DOI: https://doi.org/10.14405/kjvr.2016.56.2.125 Published online July 7, 2016.

Isolation and molecular identification of Vibrio harveyi from the look down fish (Selene vomer)

Jin-Woo Jun¹, Young-Ran Lee¹, Sib Sankar Giri¹, Hyoun-Joong Kim¹, Sae-Kil Yun¹, Cheng Chi¹, Sang-Guen Kim¹, Jeong-Rack Koh², Ji-Yun Jung², Byeong-Chun Lee¹, Se-Chang Park¹

¹College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University
²Lotte World Aquarium

Abstract

Gradual mortality of look down fish (Selene vomer) was observed in a private aquarium in Seoul, showing abnormal swimming behavior and lethargy. A bacterial pathogen from kidney was cultured, identified, and confirmed as Vibrio harveyi using Vitek System 2 and 16S rRNA gene sequencing. A predominant bacterial strain, SNUVh-LW2 was proved to be most closely related to isolates from China by phylogenetic analysis with minimum evolution method. Also, tetracycline was considered as the most sensitive antibiotic agent via antibiotic susceptibility test. The group of fish was treated according to the diagnostic result and no more mortality was observed.

Key Words: Vibrio harveyi, aquarium, look down fish