| Genomic characteristics of natural recombinant infectious bronchitis viruses isolated in Korea |
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Hyun-Woo Moon, Haan Woo Sung, Hyuk Moo Kwon |
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Laboratory of Veterinary Microbiology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University |
Correspondence:
Hyuk Moo Kwon, Tel: 82-33-250-8652, Fax: 82-33-254-5625,
Email: kwonhm@kangwon.ac.kr
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| Abstract |
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Two infectious bronchitis virus (IBV) K046-12 and K047-12 strains were isolated and the nearly complete genomes of them were sequenced. Sequence comparisons showed that the K046-12 genome was most similar to Korean IBV strains, and the K047-12 genome was most similar to QX-like IBV strains. Phylogenetic analysis showed that nearly all K046-12 and most K046-12 genes were placed in the same cluster as Korean IBV isolates, but the S1 region was placed in the same cluster as Mass-type IBVs. For K047-12, nearly all K047-12 and most K047-12 genes were located in the same cluster as QX-like IBVs, but the M region was located in the same cluster as Korean IBV isolates with K047-12. Recombination analysis confirmed that K046-12 is a recombinant strain with the primary parental sequence derived from Korean IBVs and minor parental sequence derived from Mass-type IBV, and K047-12 is a recombinant strain with the major parental sequence derived from QX-IBV and minor parental sequence derived from Korean IBVs. This study showed that new IBV recombinants are constantly generated among various IBVs, including those used for vaccination. Therefore, genetic analysis of new virus isolates should be performed for effective infectious bronchitis control and appropriate vaccine development. |
| Key Words:
infectious bronchitis virus, recombinant, sequence, phylogenetic trees |
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