Comparative Anatomy of the Korean Native Goat 2. Mesenteric mast cell

Lee, Heung Shik; Kim, Chang Ki

Korean Journal of Veterinary Research 1974;14(2):151-157.

¹Department of Anatomy, School of Medicine, Kyung Hee University
²Department of Animal Husbandry, Chungbug National College

한국 재래 산양의 비교해부학적 연구 2. 장간막 비만세포에 대하여

이흥식¹, 김창기²

¹경희대학교 의과대학 해부학교실
²충북대학 축산학과

Abstract

This study was carried out to investigate on the morphology, distribution and stainability of the mast cells in the Korean Native goat. For the study, the experimental animals were anesthetized with pentobarbital sodium and opened the anterior abdomianl wall to remove immediately the specimens with a minimum of mechanical effects. The mesenteries were fixed in 10% neutral formalin, 4% basic lead acetate, absolute alcohol and ethlene glycol monoethyl ether. Following 24 hours of fixation, the toto preparation stained with 0.4% toluidine blue, 1% methylene blue, 1.5% bismark brown, saturated thionine and thionlne-methylene blue complex solution. The preparation were observed from 10 microscopic field with 450 magnification. The results were as follows: 1. The form of the mesenteric mast cell was found 2 types. One was spindle form in larger number around vessels, the other was ovoid or spherical form in connective tissue far from blood vessels. 2. The average size was $18.63{pm}5.75{mu}m$ in length, $10.61{pm}3.39{mu}m$ in width and number was $105.50{pm}18.45$. 3. Ethylene glycol monoethyl ether was particularly useful in preserving the mast cell granules. 4. Thione-methylene blue complex solution might be recommended to stain of granules.