Korean J Vet Res > Volume 56(1); 2016 > Article
Korean Journal of Veterinary Research 2016;56(1):1-7.
DOI: https://doi.org/10.14405/kjvr.2016.56.1.1    Published online May 2, 2016.
Assessment of probiotic potential of Lactobacillus reuteri MD5-2 isolated from ceca of Muscovy ducks
Chuchat Kamollerd1, Preeyaporn Surachon1, Punchompoo Maunglai1, Wilailak Siripornadulsil2, Peerapol Sukon1
1Faculty of Veterinary Medicine, Khon Kaen University
2Faculty of Science, Khon Kaen University
Lactic acid bacteria (LAB) are commonly used as probiotics in poultry. The present study employed in vitro and in vivo methods to select and test LAB isolated from Muscovy duck ceca as potential probiotics. In the in vitro study, 50 LAB were isolated from Muscovy duck ceca and tested for growth inhibition against Salmonella (S.) Enteritidis. Eleven isolates strongly inhibited S. Enteritidis and only 1 isolate (MD5-2) showing the strongest inhibition was selected for identification. This isolate was called as Lactobacillus (L.) reuteri MD5-2. For the in vivo investigation, 90 1-day-old Muscovy ducks were randomly assigned into three groups of 30 animals each (group 1, control; group 2, treated with $10^8$ colony-forming unit (CFU) of L. reuteri MD5-2 orally once on day 1; and group 3, treated with $10^8CFU$ of L. reuteri MD5-2 orally once daily from days 1 to 5). The ducks were housed in three large cages and raised for 50 days, after which body weight, duodenal villus height and crypt depth were measured. Both villus height and villus height to crypt depth ratio were significantly greater in group 3 than in groups 1 and 2. In conclusion, further investigation of L. reuteri MD5-2 as a potential probiotic strain is warranted.
Key Words: Lactobacillus reuteri, Muscovy ducks, Salmonella Enteritidis, lactic acid bacteria, probiotics

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