Phytomitogen Induced Blast Transformation of Guinea Pig : I. Effect of Medium, Phytomitogen and Cell Concentration on the Uptake of <sup>3</sup>H-Thymidine

Kim, Jong-shu; Bak, Ung-bok

Korean Journal of Veterinary Research 1986;26(2):245-249.

Phytomitogen Induced Blast Transformation of Guinea Pig : I. Effect of Medium, Phytomitogen and Cell Concentration on the Uptake of ³H-Thymidine

Jong-shu Kim¹, Ung-bok Bak²

¹Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University
²College of Veterinary Medicine, Seoul National University

Phytomitogen에 의한 기니픽 임파구의 Blast Transformation : I. 유사분열에 미치는 배지, 유사분열촉진물질 및 세포농도의 효과

김종수¹, 박응복²

¹경상대학교 농과대학 수의학과
²서울대학교 수의과대학

Abstract

The present study has been carried out to investigate the optimal condition on the blastogenesis of guinea pig lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vito optimal condition of guinea pig lymphocytes. Data were presented to show many variables that are Involved in studying the responses of guinea pig lymphocyte in a microculture system to the stimulation of Concanavalin A(Con A) and lipopolysaccharide (LPS). Analysis indicated that the conditions for optimal Con A as measured by incorporation of $^3H$-TdR include : (1) use of RPMI-1640 as culture medium, (2) use of $6{mu}g$ of Con A, per culture, (3) use of $1{ imes}10^6$ cells per culture. Conditions for optimal stimulation with LPS mitogen were similar to those used for Con A.