Study on Antiestrogenic Effects of Tamoxifen in Immature Rat Uterus: III. Effects on Content of DNA and Protein and Uterine Wet Weight

Lee, Hyo-jong; Jo, Choong-ho; Park, Moo-hyun

Korean Journal of Veterinary Research 1987;27(1):9-17.

Hyo-jong Lee¹, Choong-ho Jo², Moo-hyun Park¹

¹Department of Veterinary Medicine, Gyeongsang National University
²College of Veterinary Medicine, Seoul National University

미성숙 쥐 자궁에서 Tamoxifen의 Antiestrogen 효과에 관한 연구 : III. DNA 함량, 단백질 함량 및 자궁의 중량에 관하여

이효종¹, 조충호², 박무현¹

¹경상대학교 농과대학 수의학과
²서울대학교 수의과대학

Abstract

The present study has been carried out to elucidate the antiestrogenic effects of tamoxifen in immature rat uterus. The content of DNA and protein and uterine wet weight were measured after the injections of $5{mu}g$ of estradiol-$17{eta}$, $50{mu}g$ of tamoxifen, a combination of both, or vehicle only subcutaneously. The results obtained were summarized as follows: 1. DNA content in uterus was increased at 48 hours after estradiol-$17{eta}$ or tamoxifen injection (p<0.01). 2. The increament rate of uterine DNA content was significantly (p<0.01) lower in tamoxifen treated group than that in estradiol-$17{eta}$ treated group. 3. Antiestrogenic effect of tamoxifen on protein content in uterus was apparent at 72 hours after simultaneous administration of both drugs. 4. The uterine wet weight was started to increase at three hours after the injection of estradiol-$17{eta}$ or tamoxifen. 5. While estradiol-$17{eta}$ increased steadily uterine wet weight up to 138.5mg at 72 hours after the injection, but tamoxifen failed to increase it after 48 hours. Tamoxifen inhibited significantly (p<0.01) the effect of estradiol-$17{eta}$ on it thereafter.