Comparative Study on the Production of Interferons from Porcine Blood Leukocytes

Han, Su-nam; Lee, Jang-nag; Lee, Chang-eop

Korean Journal of Veterinary Research 1987;27(2):191-200.

Su-nam Han, Jang-nag Lee, Chang-eop Lee

College of Veterinary Medicine, Seoul National University

돼지의 백혈구 인터폐론 생산에 관한 비교연구

한수남, 이장락, 이창업

서울대학교 수의과대학

Abstract

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${alpha},;{eta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.