Monoclonal Antibody Production against Piglet Diarrhea Agent (Enterotoxigenic E. coli) by Cell Fusion-Hybridoma Cell Technique

Kim, Uh-ho; An, Soo-hwan; Yoon, Young-dhuk

Korean Journal of Veterinary Research 1987;27(2):259-267.

Uh-ho Kim¹, Soo-hwan An², Young-dhuk Yoon²

¹Department of Animal Science, College of Agriculture, Kangweon National University
²Veterinary Research Institute, Rural Development Administration

세포융합(細胞融合) 및 Hybridoma 세포작성(細胞作成)에 의한 항자돈백리(抗仔豚白痢) Monoclone항체(抗體)의 생산(生産)

김우호¹, 안수환², 윤용덕²

¹강원대학교 농과대학 축산학과
²가축위생연구소

Abstract

Enterotoxigenic E. coli (ETEC) cause an acute diarrhea (white scour) in both animals and humans. The disease process initially involves the adherence and colonization of the mucosal surface of the small intestine, followed by the elaboration of a heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). Intestinal adherence or colonization by ETEC is generally mediated by a specific surface-associated pilus (fimbrial) antigen that endows the bacteria with the capacity to adhere to epitherial cell surface. Fourteen monoclonal antibodies (MAbs) directed against pili antigens of ETEC were obtained by cell fusion/hybridoma technique. They were characterized by indirect immunofluorescence assay (IFA), and divided into four groups: specific to K99 antigen (group 1), cross-reactive with K99 and F41 antigens (group 2), specific to K88 antigen (group 3) and specific to 987P and K88 antigens (group 4), respectively. These MAbs demonstrated the distinct pili (K) antigens on the surface of ETEC by IFA, and could be utilized as diagnostic reagent for the identification of ETEC. When eighty-seven field isolates of E. coli from piglet with diarrhea were tested by group 3 MAb, fourty-two strains (48.3%) has K88 pilus antigen suggesting that this is one of the major pilus antigen of ETEC present in fifeld.