Isolation and Identification of Porcine Rotavirus from Piglets with Diarrhoea |
Hee-sun Kim1, Chung-ok Choi1, Doo-hee Kim2, Young-soo Lyoo2, Soo-hwan An2 |
1College of Veterinary Medicine, Chonnam National University 2Veterinary Research Institute, Rural Development Administration |
하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定) |
김희선1, 최정옥1, 김두희2, 유영수2, 안수환2 |
1전남대학교 수의과대학 2가축위생연구소 |
|
Abstract |
Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera. |
|