Genentic properties of R plasmids in Salmonella isolates of swine and bovine origin in Korea II. Incompatibility and profile of R plasmid

Choi, Won-pil; Lee, Hi-suk; Yeo, San-geon; Lee, Hun-jun; Jung, Suk-chan

Korean Journal of Veterinary Research 1989;29(2):59-67.

Won-pil Choi¹, Hi-suk Lee¹, San-geon Yeo², Hun-jun Lee³, Suk-chan Jung⁴

¹College of Veterinary medicine, Kyungpook National University
²College of Veterinary medicine, Gyeonsang National University
³Department of Veterinary medicine, College of Agriculture, Chungnam National University
⁴Veterinary Research Institute, Rural Development Administration

우(牛), 돈(豚)에서 분리(分離)한 Salmonella유래(由來) R plasmid의 유전학적(遺傳學的) 및 분자생물학적(分子生物學的) 성상(性狀)에 관한 연구(硏究) II. R plasmid의 비적합성(非適合性) 및 plasmid profile

최원필¹, 이희석¹, 여상건², 이헌준³, 정석찬⁴

¹경북대학교 수의과대학
²경상대학교 수의과대학
³충남대학교 농과대학 수의학과
⁴농촌진흥철 가축위생연구소

Abstract

This paper deals with the genetic properties of R plasmids in Salmonella originated from pigs and cattle. The plasmid DNA was examined for incompatibility, stability and fertility inhibition(Fi), and gel electrophoresis was performed for isolation of plasmid DNA. The results obtained were summerized as follows: 1. Among the 66 conjugative R plasmids from 44 pigs and 22 cattle, 61 R plasmids (92.4%) were $Fi^-$, whereas the remainder were $Fi^+$. 2. The Inc groups of 66 R plasmids were determined with 7 standard plasmids. Twenty-six R plasmids were classified into Inc group $I{alpha}$, H1, H2 or F1, 40 R plasmids being not classified with standard plasmids used, and the Inc group $I{alpha}$ (57.7%) was most frequent. 3. Inc groups $I{alpha}$, H1, and F1 were identified in strains from swine, Inc groups H2 and F1 from cattle. 4. The plasmid DNA profiles in 16 Salmonella isolated from pigs and cattle were confirmed as being 1 to 10 fragments by the gel eletrophoresis. Their molecular weight ranged 1.0 to 90 megadalton. 5. The molecular weight of conjugative plasmids ranged 1.0 to 80 megadalton in 4 Salmonella (P-4, P-5, P-7 and P-8) isolated from pigs.

Key Words: Salmonella, R plasmid, incompatibility, fertility inhibition, plasmid profile