Korean Journal of Veterinary Research 1989;29(3):245-251.
Electrophysiological study on the presence of the electrogenic Na pump of the mouse unfertilized eggs
Seong-geun Hong
College of Medicine, Gyeongsang National University
Mouse 미수정란에서의 electrogenic Na pump 활동여부에 관한 연구
홍성근
경상대학교 의과대학
Abstract
In order to manifest the presence of Na-K pump and its property on the unfertilized egg membranes of mouse, membrane potential was recorded under the physiological condition (at $37^{circ}C$ and 4mM $Ca^{2+}$). After an induction of superovulation, the fresh eggs with zona pellucida were collected from mouse oviduct. Transient hyperpolarization as pump action was recorded after the switch into the high potassium perfusate (15mM $K^+$) from K-free perfusate, and the difference between membrane potential observed just before the perfusion of high potassium solution and the maximal membrane potenlial during the perfusion of high potassium solution was regard as pump activities. The results observed were as follows, 1. Resting mombrane potential was depolarized under the treatment of $10^{-5}M$ ouabain. 2. Pump activities of the unfertilized mouse eggs were $-3.38{pm}0.61mV$ ($Mean{pm}SD$, n=6), recorded as transient hyperpolarization due to the electrogenic property. 3. Pump activities were blocked by both treatment of $10^{-5}M$ ouabain and perfusion of Nafree solution, while increased by high $Na^+$ (300mM) perfusion ($-7.45{pm}0.75mV$, n =2). 4. Hyperpolarization due to pump activity was not altered by $Mn^{2+}$. 5. Above results confirm the presence of ouabain-sensitive Na-K pump, which affected the membrane potential directly, on the unfertilized egg membranes of mouse.
Key Words: unfertilized egg, Na-K pump, electrophysiological study, membrane potential, mouse


About
Browse articles
For contributors
Policy
Editorial Office
#401-1, 85 Bldg., College of Veterinary Medicine, Seoul National University
1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea
Tel: +82-2-880-1229    Fax: +82-2-878-9762    E-mail: jvs@ksvs.or.kr                

Copyright © 2024 by The Korean Society of Veterinary Science.

Developed in M2PI

Close layer
prev next