| Specificity of cell-mediated immunity in guinea pigs sensitized with Mycobacterium spp using the leukocyte adherence inhibition test |
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Seong-kuk Park1, Moo-hyung Jun1, Hun-jun Lee1, Won-gi Min1, Yong-dhuk Yoon2 |
1Department of Veterinary Medicine, College of Agriculture, Chungnam National University
2Veterinary Research Institute, Rural Development Administration |
| Leukocyte adherence inhibition test를 이용한 Mycobacterium 속균(屬菌) 감작(感作)기니픽의 세포면역반응(細胞免疫反應)의 특이성(特異性) |
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박성국1, 전무형1, 이헌준1, 민원기1, 윤용덕2 |
1충남대학교 농과대학 수의학과
2농촌진흥청 가축위생연구소 |
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| Abstract |
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In order to measure in vitro cell mediated immunity in the guinea pigs sensitized with the killed bacilli of Mycobacterium bovis ($AN_5$), M avium (serotype 2), M tuberculosis and M intracellulare (serotype 8), leukocyte adherence inhibition (LAI) test was established using the antigens of purified protein derivatives (PPD) tuberculin. By using LAI test, specificity of cell-mediated immune responses of the guinea pigs inoculated with various Mycobacterium spp was investigated, and comparison between values of LAI and skin test was also made to evaluate the specificity of the newly designed test. The results obtained throughout the experiments were summarized as follows; 1. The optimal concentration of PPD antigens for LAI test was 1 to 2mg per ml of medium. 2. When the leukocytes of guinea pigs sensitized with both M bovis($AN_5$) and M avium (serotype 2) for 2 to 8 weeks were incubated with homologous or heterologous PPD antigens, mean values of LAI test were $61.2{pm}11.2$ and $65.6{pm}5.1%$ in homologous PPD antigens respectively, while $30.0{pm}3.7$ and $32.8{pm}5.7%$ in heteNlogous PPD antigens, showing the prominently high value of LAI in the homologous syst,em (p<0.01). 3. When the leukocytes of guinea pigs sensitized with both M tuberculosis and M intracellulare (serotype 8) for 2 to 8 weeks were incubated with homologous and heterologous PPD antigens, mean values of LAI test were $67.9{pm}2.9$ and $66.9{pm}5.0%$ in homologous PPD antigens, while $27.4{pm}7.4$ and $24.4{pm}7.1%$ in heterologous PPD antigens, showing the prominently high value of LAI in the homologous system (p<0.01). 4. Comparing with the specificity of LAI and skin tests on the basis of the value obtained from the homologous system, deviation of reaction was revealed to be 49.5 to 100.2 in LAI test, and -15.9 to 52.0 in skin test. |
| Key Words:
cell-mediated immunity, Mycobacterium spp., leukocyte adherence inhibition test |
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