| Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against Sendai virus, mouse hepatitis virus and Mycoplasma pulmonis in mice and rats |
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Yoo-yeul Chung1, Hak-cheul Lee2, Eun Lee2, Byung-sam Yoo2 |
1Department of Agriculture, Korea Air and Correspondence University
2College of Agriculture and Animal Science, Yeungnam University |
| 마우스 및 랫트의 Sendai virus, mouse hepatitis Virus, Mycoplama pulmonis 감염(感染)에 대한 보체결합반응(補體結合反應)과 효소표식면역흡착측정법(酵素標識免疫吸着測定法)과의 비교(比較) |
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정유열1, 이학철2, 이은2, 유병삼2 |
1한국방송통신대학 농학과
2영남대학교 농축산대학 |
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| Abstract |
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This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine(mice and rats) antibodies against hemagglutinating virus of Japan(HVJ), mouse hepatitis virus(MHV) and Mycoplasma pulmonis(Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immunosorbent assay(ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats. |
| Key Words:
serodiagnosis, murine antibodies, immunoglobulin |
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