Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk

Kim, Min-kyu; Shin, Hyun-ju; Lee, Man-hwai; Lee, Myung-heon; Kim, Sang-keun

Korean Journal of Veterinary Research 1990;30(1):35-40.

Min-kyu Kim, Hyun-ju Shin, Man-hwai Lee, Myung-heon Lee, Sang-keun Kim

Department of Veterinary Medicine, College of Agriculture Chungnam National University

Progesterone EIA-kit에 의한 유우(乳牛)의 임신진단(姙娠診斷)에 관한 연구(硏究)

김민규, 신현주, 이만휘, 이명헌, 김상근

충남대학교 농과대학 수의학과

Abstract

This study was carried out to evaluate khe accuracy of the early diagnosis of pregnancy in dairy cows using EIK-kit of progesterone in milk and to compare the progesterone concentrations in milk with those assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows($2.07{pm}0.54ng/ml$) were significantly higher than those of non-pregnant cows($1.04{pm}0.19ng/ml$), and thereafter began to inerease and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 93.7% for non-pregnant cows, and 91.6% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 100% for non-pregnant cows (<2.7ng/ml), and 92.9% for pregnant cows($${geq_-}3.2ng/ml$$). The average overall accuracy of prediction for pregnant and non-pregnant cows were 96.5%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.

Key Words: pregnancy, EIA-kit, progesterone, dairy cows