Pathogenicity, hemagglutinability, and the effect of physicochemical agents on virus of rabbit hemorrhagic disease

Yoon, In-joong; Jeon, Yun-seong

Korean Journal of Veterinary Research 1990;30(1):65-71.

In-joong Yoon, Yun-seong Jeon

College of Veterinary Medicine, Seoul National University

토끼 출혈성 바이러스의 병원성, 적혈구응집성 및 물리화학적 요인에 대한 영향

윤인중, 전윤성

서울대학교 수의과대학

Abstract

Rabbits were experimentally infected with rabbit hemorrhagic disease virs and the viral pathogenicity, hemagglutinability, and the effect of physicochemical factors were studied. The experimental results were summariaed as follows: 1. Mean rectal temperature of 11 infected rabbits was $40.0{pm}0.47^{circ}C$ prior to the virus inoculation, and $39.9{pm}0.75^{circ}C$ after 12hrs., $40.2{pm}0.65^{circ}C$ after 24hrs., $40.1{pm}0.77^{circ}C$ after 36hurs, and $40.6{pm}0.56^{circ}C$ just before the death. 2. Mean death time of infected rabbits was $40.3{pm}22.0$ honrs and its range was 24 to 93 hours. 3. O, B, AB and A type of human erythrocytes were shown their HA in the order, but rabbit and chicken erythrocytes were not hemagglutinated by the virus. 4. In the hemagglutination, less than 0.25 per cent of a final concentration of erythrocytes and 0.2 per cent of BSA in PBS resulted in the best hemagglutination. Phosphate concentration in a range of 0.01M to 0.10M in PBS was not influenced on the hemagglutination reaction, and its pH 7.0 resulted in a better HA. 5. The hemagglutinating titers, in log 2 scale, of organs and tissues of the virus infected rabbits were $9.3{pm}3.8$ (liver), $9.1{pm}3.9$ (blood), $6.2{pm}2.6$ (spleen) and $5.0{pm}2.5$ (kidney). 6. The physicochemical factors such as heating ($50^{circ}C$, 10 min.), trypsin treatment (0.05 pre cent, 5 min.), acid treatment (pH 3.0, 20 min.) and ether extraction (3 times) were not affective to the stability of virus and viral HA activities.

Key Words: rabbit hemorrhagic virus, pathogenicity, hemagglutination, phisicochemical agents