| Studies on the improvement of reproductive efficiency in Korean native cows -Development of radioimmunoassay for progesterone- |
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Han-sun Choi, Byong-kyu Kang, Chung-gill Lee, Chang-ho Son |
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College of Veterinary Medicine, Chonnnam National University |
| 한우(韓牛)의 번식효율(繁殖效率) 증진(增進)에 관한 연구(硏究) -Progesterone측정(測定)을 위한 방사선면역분석법(放射線免疫分析法)의 개발(開發)- |
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최한선, 강병규, 이정길, 손창호 |
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전남대학교 수의과대학 |
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| Abstract |
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Radioimmunoassay for progesterone in Korean native cows was developed using the anti-4-pregnen-$11{alpha}$-ol-3,20-dione-hemisuccinate bovine serum albumin as an antibody. The final dilution of antibody was 1 : 5,000. The percentage recoveries of [$^3H$] progesterone was $83.5{pm}1.6$. The sensitivity of the assay was 8 pg per ml. The intra- and interassay coefficients of variation for the quality controls were 6.2 and 10.4%, respectively. This radioimmunoassay can be applied to puberty, estrus dectection, pregnancy diagnosis, postpartum period and diagnosis of reproductive disorders in domestic animals. |
| Key Words:
progesterone, cross-reaction, antibody, Korean native cow |
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