Protein A of Staphylococcus hyicus subsp hyicus isolated from pigs |
Do-kyoung Kim, Sang-geon Yeo |
College of Veterinary Medicine, Gyeongsang National University |
돼지에서 분리한 Staphylococcus hyicus subsp hyicus의 protein A |
김도경, 여상건 |
경상대학교 수의과대학 |
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Abstract |
The Presence and quantity of protein A in Staphylococcus hyicus subsp hyicus isolates from pigs were determined by indirect hemagglutination test and enzyme-linked immunosorbent assay(ELISA). Cell-bound and extracellular protein A was demonstrated in 87.7% and 36.0% of 489 isolates, respectively, by indirect hemagglutination test. When contents of the protein A were estimated by ELISA method, all of the isolates that were positive to protein A in indirect hemagglutination test produced extracellular protein A of less than 1ng/ml. Most of the isolates produced cell-bound protein A of less than 1ng/ml, whereas 28 isolates produced 1 to 35ng/ml and 11 isolates produced 25 to 108ng/ml. |
Key Words:
pigs, Staphylococcus hyicus subsp hyicus, protein A, indirect hemagglutination, enzyme-linked immunosorbent assay |
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